The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Antihucd is a rabbit polyclonal antibody that stains inner retinal. Further information about each individual vimentin antibody is available by following the associated links. Vimentin can be phosphorylated by akt1, a kinase activated downstream of ras and pi3k, and phosphorylation of vimentin protects it from caspaseinduced proteolysis and enhances cell migration and metastasis. If you continue, well assume that you are happy to receive all cookies. Islands of hn2 cells form after 4 days in culture forming beautiful flower like structures. Lowpower immunofluorescence image of a 24h zebrafish embryo tail somites 1830 using anti.
The vimentin antibody has been validated for the following applications. Therefore, vimentin is critical for proper organell anchoring and cellular structure. Sep 08, 2016 it was found that cells without vimentin are extremely delicate when disturbed with a micropuncture. Myc and fgf are required for zebrafish neuromast hair cell. Ovarian and uterine corpus endometrioid adenocarcinomas have different patterns of vimentin expression. Among all these vimentin antibodies, there are 2 anti vimentin rabbit monoclonal antibodies, 3 anti vimentin rabbit polyclonal antibodies. Compared to the fish in the control group, the f1 zebrafish were prematurely hatched at 48. E nvclbegfp fusion protein expression detected with a gfp antibody in.
The kit contains enough primary and secondary antibody. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Wildtype wt and vimentin knockout ko 293t cell extracts 30. A guide for the laboratory use of zebrafish danio rerio. We report a zebrafish genetrap vinculin b vclb mutant that displays abnormal. Evaluating the effects of various decalcification protocols on. This antibody recognizes human, mouse, rat antigen. Access journals video booksebooks trade magazines enewsletters. Mice lacking vimentin develop and reproduce without an. Has the antibody been tested for crossreactivity with endogenous igg.
For western blots, incubate membrane with diluted primary antibody in 5% wv nonfat dry milk, 1x tbs, 0. Vimentin antibody v9 scbt santa cruz biotechnology. Aldh1a2positive epicardial cells, two to three extra layers of vimentinpositive cells. The development of f1 zebrafish produced by the parent fish exposed to mclr is shown in fig. All the vimentin anbodies are produced in house and all are in stock. There are 3 vimentin antibody for wb, 4 vimentin antibody for ihcp.
Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Arg66199 antivimentin antibody sqab1721 iccif image. Some steps, like fusion before striation, seem to be different in the zebrafish when compared. Transglutaminasemediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Is expressed in several structures, including axis. Using a zebrafish lateral line neuromast hair cell regeneration model, we show. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. We use cookies to enhance the usability of our website.
Please use this form to recommend updates to the information in zfin. We thank dr hans heid and professor harald herrmann german cancer research center, heidelberg, germany for providing antiperilipin antibody and human vimentin cdna. Hela cells were fixed with 4% paraformaldehyde for 30 min at rt, permeabilized with 0. Oct 29, 2012 it was initially shown in 2001 that heart transplant patients produce igm anti vimentin antibodies within 2 years of transplantation and that higher titres in the first two years were predictive of cardiac allograft vasculopathy cav at 5 years.
Vimentin is a 54 kda nucleus proteincoding gene expressed in mesenchymal cells. Vimentin intermediate filament rings deform the nucleus during the first steps of adhesion. The hybridomas are stored at the monoclonal antibody. This monoclonal antibody reacts with the intermediate filament protein vimentin present in cells of mesenchymal origin. Phosphorylation of vimentin at ser56 in smooth muscle cells regulates structural arrangement of vimentin filaments in response to serotonin 5,6. Vimentin is a strong arterial substrate for transglutaminases. Your search returned 2096 vimentin antibodies across 61 suppliers. Along with actin microfilaments and tubulin microtubules, vimentin is the third major constituent of the cytoskeleton. Antibodies to vimentin can be used to study sarcoma tumor markers. Wash three times for 5 min each with 15 ml of tbst. Role of antivimentin antibodies in allograft rejection. Zebrafish antibodies primary antibodies sigmaaldrich. The fate of human malignant melanoma cells transplanted. In this study igm antibody was detected but not igg antibodies and none of the patients had ava.
As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in. Vimentin is a structural protein that in humans is encoded by the vim gene. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. The vimentin v9 antibody from santa cruz biotechnology, inc. We appreciate as much detail as possible and references as appropriate. In the embryonic nervous system, however, vimentin. Myc and fgf are required for zebrafish neuromast hair cell plos. Myc and fgf are required for zebrafish neuromast hair cell regeneration. Its name comes from the latin vimentum which refers to an array of flexible rods.
Hardcopies of the 4th edition of the zebrafish book can be obtained for. Libraries of zebrafishspecific antibodies are now available, but few. Vimentin elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vinculin b deficiency causes epicardial hyperplasia and coronary. The zebrafish book a guide for the laboratory use of. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the pns, and by neural stem cells of the cns. The kit contains enough primary and secondary antibody to perform two western blot experiments. Due to their striking genomic similarity to humans, regenerative. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns obtained with particular clones. Mouse vimentin antibody for immunostaining was from sigma v5255. The intermediate filament network changes from being composed of vimentin to. Human vimentin is a 53,521 dalton protein 466 amino acids expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton.
Vimentin antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, flow cytometry fc, enzymelinked immunosorbent assay elisa applications. Vimentin specificity is shown by immunocytochemistry in knockout cell line. The exact position of epitope sequence recognized by anti vimentin antibody clone rv202 is not determined however, the antibody must recognize an evolutionary highly conserved epitope. A monoclonal antibody stains radial glia in the adult zebrafish danio rerio cns. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Broad interspecies crossreactivity has been observed with human, rat, and chicken vimentin. The zebrafish book, in a guide for the laboratory use of zebrafish danio. Vimentin antibody protein level used designations for anti vimentin vim antibodies vimentin, vimentin, vimentin 1, vimentin 12, classiii intermediate microfilament gene id species. All together, these data support a novel mechanism by which vimentin interferes with a mirdependent negative regulation of tf mrna, thereby promoting coagulant activity. The exact position of epitope sequence recognized by anti vimentin antibody clone rv202 is not determined however, the antibody must recognize an evolutionary highly conserved epitope, since it shows a broad spectrum of species cross reactivity from human to zebrafish.
Vimentin v9 is a mouse monoclonal antibody raised against purified vimentin from eye lens of porcine origin. Vimentin, also known as ctrct30, is the full name of vim. Vimentin is an intermediate filament protein, expressed exclusively by mesenchymal cells. As an organizer of a number of critical proteins, vimentin function involved in attachment, migration, and cell signaling. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Higher magnification images of human melanoma cell stained with vimentin antibody in the 3. Vimentin antibody nbp27 whole cell extracts 30 ug was separated by 10% sdspage, and the membrane was blotted with vimentin antibody at a dilution of 1. A 28h wholemount zebrafish embryo labeled with an antibody which recognizes the transcription factor, pax6 brown label showing a lateral view with anterior to the left. Vimentin antibody customerized service is available. Vimentin was detected in immersion fixed k562 human chronic myelogenous leukemia cell line but is not detected in vimentin knockout ko k562 human cell line cell line using goat antihuman vimentin antigen affinitypurified polyclonal antibody. Global supplier of antibodies, proteins and elisa kits 25 years expertise in serving research and diagnostic customers. I am planning on using mouse monoclonal vimentin antibody 2d1, nbp192687 on mouse fibroblasts. Genetex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, with more than 400 newly developed zebrafish antibodies. If thawed upon arrival, please keep antibodies in the refrigerator at 4c.
Vimentin antibody staining protocol for immunohistochemistry. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Ihc staining of zebrafish for cytokeratin is impacted by fixative or length of. A monoclonal antibody stains radial glia in the adult zebrafish. Incubate membrane and primary antibody at the appropriate dilution and diluent as recommended in the product datasheet in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4c. Compare anti vimentin antibody products from leading suppliers on biocompare. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio. Ma511883 targets vimentin in ihc p applications and shows reactivity with bovine, canine, chicken, equine, feline, gerbil, hamster, human, mouse, nonhuman primate, porcine, rabbit, and rat samples. Wb of crude extract of human embryonic kidney stained with vimentin, showing a single strong clean band at 50kda and view of mixed neuronglial cultures stained with vimentin green and gfap antibody ra22101, red. The vimentin antibody sampler kit provides an economical means to detect total levels of vimentin, vimentin phosphorylated at ser56, and vimentin phosphorylated at ser82. Abcam antibodies and reagents supplier, find any antibody. Confocal microscopy of whole fish immunofluorescence with vimentin antibody highlights metastatic melanoma cells and their location within the zebrafish.
Further information about each individual vimentin antibody. Immunofluorescence staining of hela cells with antibody to reveal vimentin containing intermediate filaments in green and antibody. Vimentin is a developmentally regulated intermediate filament protein ifp found in cells of mesenchymal origin. Antivimentin antibody v9 cytoskeleton marker alexa fluor 488 ab195877 specific references 5. Please refer to primary antibody datasheet or product webpage for recommended antibody. Immunohistochemistry of wholemount and sectioned zebrafish tissue. Also, current batches of zirc antibody may have dilution rates that vary from the dilution rates found in publications. Please note that antibodies should be stored in a 80c freezer if they arrive frozen.
The ma511883 immunogen is purified vimentin from pig eye lens. Vimentin antibody detects endogenous levels of total human vimentin. Some distinctive features of zebrafish myogenesis based on. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. Vimentins dynamic structural changes and spatial reorganization in response to extracellular stimuli helps to coordinate various signaling pathways 4. Vim antibody mouse anti human vimentin antibody prospec. A dominant vimentin variant causes a rare syndrome with. Sectioning of embryos or larvae staining sections with antibodies using pap. Antivimentin antibody rv202 cytoskeleton marker ko. This gene encodes a member of the intermediate filament family. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate. Intermediate filamentents, along with microtubules and actin microfilaments, make up the.
Microcystinlr promotes zebrafish danio rerio oocyte in. Vimentin prevents a mirdependent negative regulation of. This antibody recognizes human, mouse, and rat antigen. It is aimed to help labome visitors find the most suited vimentin antibody.
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